Influence of gallic acid in migration ability of human gastric carcinoma SGC-7901 cells / 吉林大学学报(医学版)

Objective: To study the influence of gallic acid (GA) on the migration of human gastric carcinoma SGC-7901 cells, and to explore its mechanism.Methods: The human gastric carcinoma SGC-7901 cells were cultured and divided into control group and 3.125, 6.250, 12.500, 25.000, 50.000,100.000 mg·L-1 GA groups. The inhibitory rates of proliferation of SGC-7901 cells in various groups were examined by MTT assay;the migration abilities of SGC-7901 cells in various groups were measured with scratch assay;the expression levels of vascular of endothelial growth factor (VEGF) in various groups were detected by immunocytochemistry.Results: Compared with control group, the inhibitory rates of proliferation of SGC-7901 cells in different doses of GA groups were significantly increased in a dose-dependent manner(F=59.451,P<0.01).Compared with control group, the wound healing rates in different doses of GA groups were significantly decreased (P<0.01).Compared with control group, the expression levels of VEGF protein in 12.500 and 25.000 mg· L-1 GA groups were decreased (P<0.05).Conclusion: GA could inhibit the proliferation and migration of SGC-7901 cells through down-regulating the expression levels of VEGF protein.

A new tetrahydrofuran lignan from Peperomia blanda / 国际药学研究杂志

Objective To investigate the chemical constituents of Peperomia blanda. Methods Guided by HPLC detection, integrated methods including vacuum liquid chromatography (VLC), ODS and semi-preparative RP-HPLC were used for separation. The structure was determined by spectral analyses including ESI-MS, 1D NMR, 2D NMR and ECD spectra. Results Four compounds were isolated and identified as (7S,7’S,8R,8 ’R)-7-(5-methoxy-3,4-methylenedioxyphenyl)-7-(4-hydroxy-3,5-dimethoxy-phenyl)-8, 8-dihydroxymethyltetrahydrofuran (1), 7, 8-trans-8,8-trans-7,8-cis-7, 7-(4-hydroxy-3,5-dimethoxy phenyl)-8,8-di-acetoxymethyltetrahydrofuran (2), (+)-(7S, 7S, 8R, 8R)-4, 4-dihydroxy-3, 3, 5, 5-tetramethoxy-7, 9, 7, 9-diepoxylignane (3), and (6R, 7E, 9R)-9-hydroxy-4, 7-megastigmadien-3-one (4). Conclusion Compound 1 is a new tetrahydrofuran lignan, compounds 2-4 were isolated from P. blanda for the first time.

Thymosin Beta-4 Recombinant Adeno-associated Virus Enhances Human Nucleus Pulposus Cell Proliferation and Reduces Cell Apoptosis and Senescence / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Thymosin beta-4 (TB-4) is considered key roles in tissue development, maintenance and pathological processes. The study aimed to prove TB-4 positive biological function on nucleus pulposus (NP) cell apoptosis and slowing the process of cell aging while increasing the cell proliferation.</p><p><b>METHODS</b>TB-4 recombinant adeno-associated virus (AAV) was constructed and induced to human NP cells. Cell of same group were cultured without gene modification as controlled group. Proliferation capacity and cell apoptosis were observed during 6 passages of the cells. Morphology and expression of the TB-4 gene were documented as parameter of cell activity during cell passage.</p><p><b>RESULTS</b>NP cells with TB-4 transfection has normal TB-4 expression and exocytosis. NP cells with TB-4 transfection performed significantly higher cell activity than that at the control group in each generation. TB-4 recombinant AAV-transfected human NP cells also show slower cell aging, lower cell apoptosis and higher cell proliferation than control group.</p><p><b>CONCLUSIONS</b>TB-4 can prevent NP cell apoptosis, slow NP cell aging and promote NP cell proliferation. AAV transfection technique was able to highly and stably express TB-4 in human NP cells, which may provide a new pathway for innovation in the treatment of intervertebral disc degenerative diseases.</p>

Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens

Toxoplasma gondii is a protozoan parasite with a broad range of intermediate hosts. Chickens as important food-producing animals can also serve as intermediate hosts. To date, experimental studies on the pathogenicity of T. gondii in broiler chickens were rarely reported. The objective of the present study was to compare the pathogenicity of 5 different T. gondii strains (RH, CN, JS, CAT2, and CAT3) from various host species origin in 10-day-old chickens. Each group of chickens was infected intraperitoneally with 5 x 10(8), 1 x 10(8), 1 x 10(7), and 1 x 10(6) tachyzoites of the 5 strains, respectively. The negative control group was mockly inoculated with PBS alone. After infection, clinical symptoms and rectal temperatures of all the chickens were checked daily. Dead chickens during acute phage of the infection were checked for T. gondii tachyzoites by microscope, while living cases were checked for T. gondii infection at day 53 post-inoculation (PI) by PCR method. Histopathological sections were used to observe the pathological changes in the dead chickens and the living animals at day 53 PI. No significant differences were found in survival periods, histopathological findings, and clinical symptoms among the chickens infected with the RH, CN, CAT2, and CAT3 strains. Histopathological findings and clinical symptoms of the JS (chicken origin) group were similar to the others. However, average survival times of infected chickens of the JS group inoculated with 5 x 10(8) and 1 x 10(8) tachyzoites were 30.0 and 188.4 hr, respectively, significantly shorter than those of the other 4 mammalian isolates. Chickens exposed to 10(8) of T. gondii tachyzoites and higher showed acute signs of toxoplasmosis, and the lesions were relatively more severe than those exposed to lower doses. The results indicated that the pathogenicity of JS strain was comparatively stronger to the chicken, and the pathogenicity was dose-dependent.

Role of P38 in endoplasmic reticulum stress-induced chemoresistance to cisplatin in gastric cancer cells / 中国肿瘤临床

Objective:To investigate the mechanism of endoplasmic reticulum (ER) stress-induced chemoresistance to cisplatin in gastric cancer cells. Methods:ER stress models were established in both BGC823 and SGC7901 gastric cancer cells. The expression of GRP78, an ER stress marker, was examined by Western blot analysis. Moreover, whether ER stress can decrease the sensitivity of gastric cancer cells to cisplatin and activate P38 was explored by flow cytometry and Western blot analysis, respectively. Whether ER stress-induced chemoresistance to cisplatin can be abrogated by blocking P38 activity in gastric cancer was also elucidated using flow cytometry. Results:GRP78 protein expression markedly increased after treating BGC823 and SGC7901 gastric cancer cells with tunica-mycin (TM) or thapsigargin (TG) for 8, 16, and 24 h (P0.05). Both P38 inhibitors, either SB203580 or PD169316, can inhibit the activation of P38. The inhibition of P38 activity can overcome ER stress-induced chemoresistance to cisplatin in gastric cancer cells (P<0.05). Con-clusion:ER stress can trigger the chemoresistance to cisplatin by activating P38 in gastric cancer cells.

Effects of exhaustive exercise-induced oxidative stress on red blood cell deformability / 中国应用生理学杂志

<p><b>OBJECTIVE</b>The aim of the present study is to explore the effects of exhaustive exercise-induced oxidative stress on the antioxidant capacity and diformability of rat red blood cells.</p><p><b>METHODS</b>Rats were divided into three group (n = 10): sedentary control (C), exhaustive running exercise (ERE) and moderate running exercise (MRE) groups. Animals in the ERE group started treadmill running at a speed of 20 m/min speed with a 5% gradient, and reached a speed of 25 m/min with gradient 15% in 20 min. Running was continued until exhaustion. MRE group rats running at a speed of 20 m/min with a 5% gradient for 40 min. The levels of free thiol in erythrocyte membrane protein, lipidperoxidation levels and membrane protein components were analyzed. The red blood cell deformability of different groups was also observed.</p><p><b>RESULTS</b>The results showed that red blood cells were damaged by severe oxidative stress and the anti-oxidative capacity decreased significantly under exhaustive exercise conditions. Besides, lipid peroxidation and protein sulfhydryl cross-link based clustering of membrane were found after exhaustive exercise, and polymers high molecular weight (HMW) was formed. The elongation index (EI) was found to decline significantly in the ERE group compared with the C and MRE groups under shear stress (control group, 0.41 +/- 0.01 at 3 Pa and 0.571 +/- 0.008 at 30 Pa; ERE group, 0.314 +/- 0.013 at 3 Pa and 0.534 +/- 0.009 at 30 Pa; P < 0.05 and P < 0.01, respectively).</p><p><b>CONCLUSION</b>These exercise-induced oxidative injure result in a significant decrease in deformability of rat erythrocytes, which in turn leads to dysfunction in the microcirculatory.</p>

The rise of high intensity focused ultrasound (HIFU) non-invasive therapy for solid tumors / 中华肝胆外科杂志

In the 40 years of last centry American scientists put forward a concep of high intensity focused ultrasound (HIFU) therapeutic technique and had done some technical and clinical studies. Since 90 years the HIFU surgery technique treating tumors anew rised abruptly in the world. China firstly put out a comprehensive HIFU tumor treating sistem and successfully treated many solid tumors such as breast cancer, bone tumor, liver cancer etc.. Now the treating sistem has already exported to England, Italy,Spain, Japan, Colea etc. and treated tens thousands tumor patients. In the field of noninvasive ultrasound treating tumor technique China has continually kept the leading position in the world.

Study on therapeutic dosimetry of HIFU ablation tissue / 生物医学工程学杂志

It is a difficult problem in high intensity focused ultrasound (HIFU) therapeutic dosimetry that how to use a BFR to ablate a mass in tissue and to determine the energy-efficiency relation, that is, the scale of biological effects of HIFU. A mass lesion was realized in this study according to a treatment principle of damaging tissue from BFRs to fascicle lesions, slice lesions and a mass lesion. A 1.6 MHz transducer, 150 mm in diameter and with a focal length of 120 mm, was used. The focal intensities (I(SATA)) were 0-27 000 W/cm2 and the scanning speeds were 1-4 mm/s. The distance between every fascicle lesion was 5-10 mm and the distance between two slice lesions was 10-20 mm. Different irradiation depths of fascicle slice and mass lesion were observed after HIFU procedures in this study. The dosage of HIFU required for tissue coagulated necrosis was evaluated with energy of HIFU (J) per cubic millimeter (mm3), i.e., J/mm3 which was defined as energy-efficiency factor (EEF). Results showed that EEF needed for producing fascicle lesions increased with the increase of irradiation depth. EEF required for inducing various lesions in biological tissue was different. Generally, it followed the law: EEF(mase)< EEF(slice)<EEF(fascicle). EEF for slice lesion was not simply a summation of EEFs for fascicle lesions at different irradiation depths, although the slice lesion was assembled with fascicle lesions at different irradiation depths in the same treatment slice. In the same way, EEF for a mass lesion was not simple summation of EEFs for slice lesions at different layers. So HIFU therapeutic dosimetry can be carried on investigation by using EEF, Factors of affecting EEF of HIFU include acoustic power, exposure time, irradiation depth, tissue structure, and tissue functional status. Besides, another important factor is the change of the acoustic environment in tissue during the HIFU procedure.

Effects of therapeutic dose on temperature rise induced by high intensity focused ultrasound in tissue / 生物医学工程学杂志

Based on the well-known Pennes' bioheat equation, we analyzed theoretically the temperature rise in tissue during high intensity focused ultrasound(HIFU) irradiation, and measured the focal temperature rise induced by HIFU in the treatment of freshly excised cow liver with different acoustic intensity and exposure time. The results showed that a threshold exposure time always existed under a certain acoustic intensity. Temperature rise was slow when exposure time exceeded the threshold exposure time. The greater the acoustic intensity was, the earlier the threshold time appeared. The focal temperature rise and the relative cumulative thermal dose (RCTD) increased with the increase of acoustic intensity and exposure time. For a certain therapeutic dose, the effects of acoustic intensity on focal temperature rise were more distinct than the effects of exposure time on focal temperature rise. Therefore, the optimal HIFU therapeutic dose should meet the need, i.e. moderate acoustic intensity, and the exposure time be the threshold exposure time under this acoustic intensity.

Study of ultrasound attenuation during HIFU propagation in ox liver / 生物医学工程学杂志

Ultrasound attenuation has been measured during HIFU propagation in fresh ox liver at different frequency using a radiation force method. The acoustic radiation force was measured before and after the prepared ox liver of various thicknesses (20 mm, 40 mm, 60 mm, 80 mm) which were put into degassed water and exposed to different transducer surface output acoustic powers at a room temperature (20 degrees C) with therapeutic transducers of various frequencies, and then the ultrasound attenuation coefficient was calculated. With the use of therapeutic transducer 4, the focus of the ultrasonic beam was set to be 20 mm, 40 mm or 60 mm deep into the tissue surface using B-mode ultrasound guidance. A single exposure was performed with focal intensity ISATA = 22.0 x 10(3) W/cm2(ISATA, in degassed water) and exposure time 5 sec. The sample was cut after treatment to measure the volume of coagulative necrosis. For a specific therapeutic transducer, the radiation force ratio in liver of constant thickness is independent of the transducer surface sound intensity and the area of the ultrasound window at the sample front face. The radiation force ratio and the volume of coagulative necrosis induced by HIFU are plotted as the functions of sample thickness (or exposed depth), in which it can be seen that these two parameters have the same exponential dependence on sample thickness. The ultrasound attenuation coefficient alpha in ox liver is shown to be frequency f dependent, it almost linearly increases with frequency t. This work shows that such a study is feasible, and offers experimental data that will be useful for future HIFU dosage studies.